NPPB Protein (AA 27-102) (DYKDDDDK Tag,His tag)
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- Target See all NPPB Proteins
- NPPB (Natriuretic Peptides B (NPPB))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-102
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NPPB protein is labelled with DYKDDDDK Tag,His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- Purpose
- Recombinant Human BNP Protein is produced by E.coli expression system and the target gene encoding His27-Arg102 is expressed with a 6His, Flag tag at the N-terminus.
- Sequence
- MNHKVHHHHH HMDYKDDDDK HPLGSPGSAS DLETSGLQEQ RNHLQGKLSE LQVEQTSLEP LQESPRPTGV WKSREVATEG IRGHRKMVLY TLRAPR
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product NPPB Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- NPPB (Natriuretic Peptides B (NPPB))
- Alternative Name
- NPPB (NPPB Products)
- Synonyms
- BNP1 Protein, NPPB Protein, BNP Protein, Bnf Protein, AA408272 Protein, natriuretic peptide B Protein, natriuretic peptide type B Protein, NPPB Protein, Nppb Protein
- Gene ID
- 4879
- UniProt
- P16860
- Pathways
- Hormone Activity
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