Fibronectin 1 Protein (FN1) (AA 5-95) (His tag,AVI tag)
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- Target See all Fibronectin 1 (FN1) Proteins
- Fibronectin 1 (FN1)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 5-95
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Fibronectin 1 protein is labelled with His tag,AVI tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Mass Spectrometry (MS)
- Purpose
- Recombinant Human Fibronectin Protein is produced by E.coli expression system and the target gene encoding Glu5-Thr95 is expressed with a 6His, Avi tag at the N-terminus.
- Sequence
- MNHKVHHHHH HMGLNDIFEA QKIEWHEGGG GSEVPQLTDL SFVDITDSSI GLRWTPLNSS TIIGYRITVV AAGEGIPIFE DFVDSSVGYY TVTGLEPGID YDISVITLIN GGESAPTTLT QQT
- Purity
- Greater than 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
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Fibronectin 1 (FN1) (AA 1-163) protein (GST tag)
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Fibronectin 1 (FN1) (AA 627-702) protein (His tag)Crystallography grade FN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Fibronectin 1 (FN1) (AA 723-903) protein (His tag)Crystallography grade FN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Fibronectin 1 (FN1) (AA 627-701) protein (His tag)Crystallography grade FN1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Fibronectin 1 (FN1) (AA 723-911) protein (His tag)Crystallography grade FN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- Target
- Fibronectin 1 (FN1)
- Alternative Name
- FN1 (FN1 Products)
- Synonyms
- FN1 Protein, FN Protein, cig Protein, fibronectin Protein, finc Protein, lets Protein, msf Protein, Fn Protein, fn2 Protein, fb80d10 Protein, wu:fb80d10 Protein, CIG Protein, ED-B Protein, FINC Protein, FNZ Protein, GFND Protein, GFND2 Protein, LETS Protein, MSF Protein, E330027I09 Protein, Fn-1 Protein, FIBNEC Protein, fn-1 Protein, fibronectin 1 Protein, fibronectin 1a Protein, fibronectin 1 S homeolog Protein, FN1 Protein, fn1 Protein, fn1a Protein, Fn1 Protein, fn1.S Protein
- Gene ID
- 2335
- UniProt
- P02751
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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