IDO Protein (AA 2-403) (His tag)
IDO
Origin: Human
Host: Escherichia coli (E. coli)
> 97 %
AcA, CC
Active
2 images
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- Target See all IDO Proteins
- IDO (Indoleamine 2,3-Dioxygenase (IDO))
- Biological Activity
- Active
- Protein Characteristics
- AA 2-403
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IDO protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- IDO (Indoleamine 2,3-dioxygenase 1) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. Thus, bioactivity of recombinant human IDO was measured through its ability to oxidize L-tryptophan to N-formyl-kynurenine, using Methylene Blue as indicator. Briefly, a mixture of 800μM L-tryptophan, 9000 units/mL catalase, and 40μM Methyene Blue with the addition of 80mM ascorbic acid reaction buffer was incubated with different concentrations of IDO, and the absorbance was read in 321nm every 1.5h. The result indicated that recombinant human IDO oxidize L-tryptophan Enzyme activity of recombinant human IDO.
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Indoleamine 2,3-Dioxygenase (IDO) protein
IDO Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Indoleamine 2,3-Dioxygenase (IDO) proteinIDO Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Application Notes
- Isoelectric Point: 6.8
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % skl, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- IDO (Indoleamine 2,3-Dioxygenase (IDO))
- Alternative Name
- Indoleamine-2,3-Dioxygenase (IDO) (IDO Products)
- Synonyms
- IDO Protein, IDO-1 Protein, INDO Protein, Ido Protein, Indo Protein, indoleamine 2,3-dioxygenase 1 Protein, IDO1 Protein, Ido1 Protein
- Background
- Alternative Names: CD107B, INDO, Indoleamine-Pyrrole 2,3 Dioxygenase
- Molecular Weight
- 43kDa
- UniProt
- P14902
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