LCAT Protein (AA 41-210) (His tag)
-
- Target See all LCAT Proteins
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Biological Activity
- Active
- Protein Characteristics
- AA 41-210
-
Origin
- Mouse
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LCAT protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 90 %
- Biological Activity Comment
- LCAT (Phosphatidylcholine-sterol acyltransferase) is an enzyme in the extracellular metabolism of plasma lipoproteins, which converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines. It is reported that APOA1 (Apolipoprotein A-I) acts as a cofactor for the LCAT. Thus, a binding ELISA assay was conducted to detect the association of LCAT with APOA1. Briefly, recombinant rat LCAT were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-LCAT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of LCAT with APOA1 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of LCAT with APOA1.
- Top Product
- Discover our top product LCAT Protein
-
-
- Application Notes
- Isoelectric Point: 6.3
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- Preservative
- Dithiothreitol (DTT), ProClin
- Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- Target
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Alternative Name
- Lecithin Cholesterol Acyltransferase (LCAT) (LCAT Products)
- Synonyms
- AI046659 Protein, D8Wsu61e Protein, MGC82035 Protein, lcat Protein, MGC88964 Protein, LCAT Protein, lecithin-cholesterol acyltransferase Protein, lecithin cholesterol acyltransferase Protein, lecithin-cholesterol acyltransferase L homeolog Protein, solute carrier family 12 member 4 Protein, fragile site, aphidicolin type, common, fra(13)(q13.2) Protein, LCAT Protein, Lcat Protein, lcat.L Protein, lcat Protein, SLC12A4 Protein, FRA13A Protein
- Background
- Alternative Names: phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase
- Molecular Weight
- 22kDa
- UniProt
- P16301
- Pathways
- Lipid Metabolism
-