TNFRSF18 Protein (AA 74-199) (DYKDDDDK Tag)

Catalog No. ABIN6253216

This Recombinant TNFRSF18 protein is expressed in HEK-293 Cells.

Quick Overview for TNFRSF18 Protein (AA 74-199) (DYKDDDDK Tag) (ABIN6253216)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 74-199

Purification tag / Conjugate: This TNFRSF18 protein is labelled with DYKDDDDK Tag.

Purpose: GITRL, Soluble (human) (rec.)

Specificity: The extracellular domain of human GITRL (aa 74-199) is fused at the N-terminus to a FLAG®-tag.

Characteristics: Protein. The extracellular domain of human GITRL (aa 74-199) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.06EU/μg purified protein (LAL test, Lonza). Lyophilized from 0.2μm-filtered solution in PBS. Purity: >90 % (SDS-PAGE). GITRL (Glucocorticoid-induced TNF receptor ligand) is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g. testis). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

Endotoxin Level: <0.06EU/μg purified protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: GITRL, Soluble

Background:

Alternate Names/Synonyms: Glucocorticoid-induced TNF Receptor Ligand, AITRL, Activation-inducible TNF-related Ligand, TNFSF18

Product Description: GITRL (Glucocorticoid-induced TNF receptor ligand) is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g. testis). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

Molecular Weight: ~17kDa (SDS-PAGE)

Pathways: Cancer Immune Checkpoints