IL-1 beta Protein (AA 117-269) (His tag)

Catalog No. ABIN7278697

Recombinant IL-1 beta protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-1 beta Protein (AA 117-269) (His tag) (ABIN7278697)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 117-269

Purification tag / Conjugate: This IL-1 beta protein is labelled with His tag.

Purpose: IL-1beta (human) (rec.) (His)

Specificity: Human IL-1beta (aa 117-269) is fused at the C-terminus to a His-tag.

Characteristics: Protein. Human IL-1beta (aa 117-269) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.1EU/μg protein (LAL test, Lonza). Lyophilized from a concentrated sterile solution containing 50  mM Tris-Hcl buffer (  pH 8.0) and 100  mM NaCl. Purity: >98 % (SDS-PAGE). IL-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta belongs to the IL-1 family of proteins that are involved in the inflammatory response. IL-1beta in most situations requires the inflammasome complex to be cleaved and secreted.

Endotoxin Level: <0.1EU/μg protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from a concentrated sterile solution containing 50  mM Tris-Hcl buffer (  pH 8.0) and 100  mM NaCl.

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1beta

Background:

Alternate Names/Synonyms: Interleukin-1beta IL-1F2

Product Description: IL-1beta is produced by activated macrophages. IL-1beta stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1beta belongs to the IL-1 family of proteins that are involved in the inflammatory response. IL-1beta in most situations requires the inflammasome complex to be cleaved and secreted.

NCBI Accession: NP_000567

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome