IL1A Protein (AA 113-271) (His tag)
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- Target See all IL1A Proteins
- IL1A (Interleukin 1 alpha (IL1A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 113-271
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IL1A protein is labelled with His tag.
- Purpose
- IL-1alpha (human) (rec.) (His)
- Specificity
- Human IL-1alpha (aa 113-271) is fused at the C-terminus to a His-tag.
- Characteristics
- Protein. Human IL-1alpha (aa 113-271) is fused at the C-terminus to a His-tag. Source: E. coli. Endotoxin content: <0.1EU/μg protein (LAL test, Lonza). Lyophilized from a concentrated sterile solution containing 50 mM Tris-HCl buffer ( pH 8.0) and 100 mM NaCl. Purity: >98 % (SDS-PAGE). The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.
- Purity
- >98 % (SDS-PAGE)
- Endotoxin Level
- <0.1EU/μg protein (LAL test, Lonza).
- Top Product
- Discover our top product IL1A Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized from a concentrated sterile solution containing 50 mM Tris-HCl buffer ( pH 8.0) and 100 mM NaCl.
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- Target
- IL1A (Interleukin 1 alpha (IL1A))
- Alternative Name
- IL-1alpha (IL1A Products)
- Synonyms
- IL-1A Protein, IL1 Protein, IL1-ALPHA Protein, IL1F1 Protein, IL-6 Protein, Il-1a Protein, IL-1 alpha Protein, IL-1alpha Protein, L1A Protein, interleukin 1 alpha Protein, interleukin 6 Protein, IL1A Protein, IL6 Protein, Il1a Protein
- Background
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Alternate Names/Synonyms: Interleukin-1alpha, IL-1F1, IL-1A
Product Description: The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.
- NCBI Accession
- NP_000566
- Pathways
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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