COCH Protein (AA 25-132) (DYKDDDDK Tag)

Catalog No. ABIN6253494

Product Details

Target: COCH (Cochlin (COCH))

Protein Type: Recombinant

Protein Characteristics: AA 25-132

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This COCH protein is labelled with DYKDDDDK Tag.

Purpose: Cochlin (human) (LCCL domain) (rec.)

Specificity: Human cochlin (LCCL domain) (aa 25-132) is fused at the N-terminus to a FLAG®-tag.

Characteristics: Protein. Human cochlin (LCCL domain) (aa 25-132) is fused at the N-terminus to a FLAG®-tag. Source: HEK 293 cells. Endotoxin content: <0.01EU/μg purified protein (LAL test, Lonza). Lyophilized. Contains PBS. Purity: >95 % (SDS-PAGE). Cochlin is an extracellular matrix protein expressed in the cochlea and vestibule of the inner ear. Mutations in the cochlin gene are linked to the autosomal-dominant DFNA9 syndrome, a disorder characterized by hearing loss. Cochlin is also expressed in follicular dendritic cells of the spleen and the lymph nodes. The N-terminus of cochlin contains a LCCL domain also present in Factor C, a serine protease of the horseshoe crab Limulus, involved in activating the coagulation cascade in response to LPS. A recent study showed that in response to inflammation, LPS or bacterial infection, cochlin is cleaved by the proteases aggrecanases and secreted into blood circulation. The secreted cochlin (LCCL domain) seems to have a role in local cytokine production, recruitment of immune effector cells and bacterial clearance. Cochlin is secreted as two forms p8 and p18, whereas p18 being the glycosylated form of p8.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: COCH (Cochlin (COCH))

Alternative Name: Cochlin (COCH Products)

Synonyms: AW122937 Protein, Coch-5B2 Protein, D12H14S564E Protein, COCH-5B2 Protein, COCH5B2 Protein, DFNA9 Protein, cochlin Protein, Coch Protein, COCH Protein

Background:

Alternate Names/Synonyms: COCH-5B2 (LCCL domain)

Product Description: Cochlin is an extracellular matrix protein expressed in the cochlea and vestibule of the inner ear. Mutations in the cochlin gene are linked to the autosomal-dominant DFNA9 syndrome, a disorder characterized by hearing loss. Cochlin is also expressed in follicular dendritic cells of the spleen and the lymph nodes. The N-terminus of cochlin contains a LCCL domain also present in Factor C, a serine protease of the horseshoe crab Limulus, involved in activating the coagulation cascade in response to LPS. A recent study showed that in response to inflammation, LPS or bacterial infection, cochlin is cleaved by the proteases aggrecanases and secreted into blood circulation. The secreted cochlin (LCCL domain) seems to have a role in local cytokine production, recruitment of immune effector cells and bacterial clearance. Cochlin is secreted as two forms p8 and p18, whereas p18 being the glycosylated form of p8.

UniProt: O43405

Pathways: Sensory Perception of Sound