POLD4 Protein (AA 1-107) (His tag)
Quick Overview for POLD4 Protein (AA 1-107) (His tag) (ABIN6387269)
Target
See all POLD4 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-107
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Purification tag / Conjugate
- This POLD4 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL
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Purification
- > 90% by SDS-PAGE
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Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-107) protein (His tag)
custom-made POLD4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-107) protein (His tag)custom-made POLD4 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-107) protein (His-IF2DI Tag)POLD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-34) protein (GST tag)POLD4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) protein (Myc-DYKDDDDK Tag)POLD4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))
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Alternative Name
- DNA polymerase delta subunit 4/POLD4
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Background
- POLD4 is the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human POLD4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 14.8 kDa (130aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_066996
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Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
Target
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