POLD4 Protein (AA 1-107) (His tag)
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- Target See all POLD4 Proteins
- POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-107
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLD4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMGRKRLI TDSYPVVKRR EGPAGHSKGE LAPELGEEPQ PRDEEEAELE LLRQFDLAWQ YGPCTGITRL QRWCRAKQMG LEPPPEVWQV LKTHPGDPRF QCSLWHLYPL
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLD4 Protein
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Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-107) protein (His tag)
POLD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) (AA 1-34) protein (GST tag)POLD4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4) protein (Myc-DYKDDDDK Tag)POLD4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))
- Alternative Name
- POLD4 (POLD4 Products)
- Synonyms
- 2410012M21Rik Protein, AI463381 Protein, AW060307 Protein, Polds Protein, p12 Protein, POLDS Protein, DNA polymerase delta 4, accessory subunit Protein, polymerase (DNA-directed), delta 4 Protein, POLD4 Protein, Pold4 Protein
- Background
- POLD4 is the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Recombinant human POLD4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 14.8 kDa (130aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_066996
- UniProt
- Q9HCU8
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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