IMPAD1 Protein (AA 34-359) (His tag)
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- Target See all IMPAD1 Proteins
- IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 34-359
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This IMPAD1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK
- Purity
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific acitivty > 3300 pmole/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.
- Top Product
- Discover our top product IMPAD1 Protein
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Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-359) protein (His tag)
IMPAD1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 1-359) protein (GST tag)IMPAD1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Inositol Monophosphatase Domain Containing 1 (IMPAD1) protein (His tag)IMPAD1 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-356) protein (His tag)IMPAD1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-356) (Active) protein (His tag)IMPAD1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS Active
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4)
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))
- Alternative Name
- IMPAD1 (IMPAD1 Products)
- Synonyms
- IMP 3 Protein, RGD1306455 Protein, gPAPP Protein, impa3 Protein, 1110001C20Rik Protein, AA408880 Protein, AI451589 Protein, AL022796 Protein, B230207P20 Protein, Jaws Protein, GPAPP Protein, IMP-3 Protein, IMPA3 Protein, IMPase 3 Protein, zgc:123256 Protein, inositol monophosphatase domain containing 1 Protein, inositol monophosphatase domain containing 1 S homeolog Protein, Impad1 Protein, impad1.S Protein, IMPAD1 Protein, impad1 Protein
- Background
- IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- Molecular Weight
- 37.6kDa (349aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_060283
- UniProt
- Q9NX62
- Pathways
- Glycosaminoglycan Metabolic Process
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