IL-3 Protein (AA 20-152) (His tag)
-
- Target See all IL-3 Proteins
- IL-3 (Interleukin 3 (IL-3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 20-152
-
Origin
- Human
-
Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This IL-3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIFLEHHHHH H
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effects less or equal to 1.5 ng/ml.
- Top Product
- Discover our top product IL-3 Protein
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Bioactivity Validated
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- IL-3 (Interleukin 3 (IL-3))
- Alternative Name
- IL3 (IL-3 Products)
- Synonyms
- BPA Protein, Csfmu Protein, HCGF Protein, Il-3 Protein, MCGF Protein, PSF Protein, IL-3 Protein, MULTI-CSF Protein, interleukin 3 Protein, interleukin 3 (colony-stimulating factor, multiple) Protein, Il3 Protein, IL3 Protein
- Background
- IL3, as known as interleukin 3, is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. Also, this protein affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor, P-cell stimulation factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factors and WEHI-3 growth factor. Recombinant human IL3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 16.1kDa (141aa) 13.5-18kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_000579
- UniProt
- P08700
- Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
-