CNDP1 Protein (AA 27-507) (His tag)
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- Target See all CNDP1 Proteins
- CNDP1 (Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 27-507
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This CNDP1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- SPSPPPALLE KVFQYIDLHQ DEFVQTLKEW VAIESDSVQP VPRFRQELFR MMAVAADTLQ RLGARVASVD MGPQQLPDGQ SLPIPPVILA ELGSDPTKGT VCFYGHLDVQ PADRGDGWLT DPYVLTEVDG KLYGRGATDN KGPVLAWINA VSAFRALEQD LPVNIKFIIE GMEEAGSVAL EELVEKEKDR FFSGVDYIVI SDNLWISQRK PAITYGTRGN SYFMVEVKCR DQDFHSGTFG GILHEPMADL VALLGSLVDS SGHILVPGIY DEVVPLTEEE INTYKAIHLD LEEYRNSSRV EKFLFDTKEE ILMHLWRYPS LSIHGIEGAF DEPGTKTVIP GRVIGKFSIR LVPHMNVSAV EKQVTRHLED VFSKRNSSNK MVVSMTLGLH PWIANIDDTQ YLAAKRAIRT VFGTEPDMIR DGSTIPIAKM FQEIVHKSVV LIPLGAVDDG EHSQNEKINR WNYIEGTKLF AAFFLEMAQL HLEHHHHHH
- Purity
- > 95 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is >3,000 pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.
- Top Product
- Discover our top product CNDP1 Protein
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Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1) (AA 28-147) protein (His-IF2DI Tag)
CNDP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1) (AA 1-507) protein (GST tag)CNDP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1) (Active) protein (His tag)CNDP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE Active
Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1) (AA 1-492) protein (His tag)CNDP1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Bioactivity Validated
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- CNDP1 (Carnosine Dipeptidase 1 (Metallopeptidase M20 Family) (CNDP1))
- Alternative Name
- CNDP1 (CNDP1 Products)
- Synonyms
- cn1 Protein, cpgl2 Protein, CNDP1 Protein, CN1 Protein, CPGL2 Protein, HsT2308 Protein, AI746433 Protein, Cn1 Protein, carnosine dipeptidase 1 (metallopeptidase M20 family) L homeolog Protein, carnosine dipeptidase 1 Protein, carnosine dipeptidase 1 (metallopeptidase M20 family) Protein, cndp1.L Protein, CNDP1 Protein, cndp1 Protein, Cndp1 Protein
- Background
- CNDP1, also known as beta-Ala-His dipeptidase, belongs to the peptidase M20A family. The shortest allelic form (CNDP1 Mannheim) was more common in the absence of nephropathy and was associated with lower serum carnosinase levels. Carnosine inhibited the increased production of fibronectin and collagen type VI in podocytes and the increased production of TGF-beta in mesangial cells. Diabetic patients with the CNDP1 Mannheim variant are less susceptible for nephropathy. Carnosine protects against the adverse effects of high glucose levels on renal cells. Recombinant human CNDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 54.9kDa (489aa) 50-70KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_116038
- UniProt
- Q96KN2
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