TDP1 Protein (AA 1-608) (His tag)
-
- Target See all TDP1 Proteins
- TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-608
-
Origin
- Human
-
Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This TDP1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPMSQEGDY GRWTISSSDE SEEEKPKPDK PSTSSLLCAR QGAANEPRYT CSEAQKAAHK RKISPVKFSN TDSVLPPKRQ KSGSQEDLGW CLSSSDDELQ PEMPQKQAEK VVIKKEKDIS APNDGTAQRT ENHGAPACHR LKEEEDEYET SGEGQDIWDM LDKGNPFQFY LTRVSGVKPK YNSGALHIKD ILSPLFGTLV SSAQFNYCFD VDWLVKQYPP EFRKKPILLV HGDKREAKAH LHAQAKPYEN ISLCQAKLDI AFGTHHTKMM LLLYEEGLRV VIHTSNLIHA DWHQKTQGIW LSPLYPRIAD GTHKSGESPT HFKADLISYL MAYNAPSLKE WIDVIHKHDL SETNVYLIGS TPGRFQGSQK DNWGHFRLKK LLKDHASSMP NAESWPVVGQ FSSVGSLGAD ESKWLCSEFK ESMLTLGKES KTPGKSSVPL YLIYPSVENV RTSLEGYPAG GSLPYSIQTA EKQNWLHSYF HKWSAETSGR SNAMPHIKTY MRPSPDFSKI AWFLVTSANL SKAAWGALEK NGTQLMIRSY ELGVLFLPSA FGLDSFKVKQ KFFAGSQEPM ATFPVPYDLP PELYGSKDRP WIWNIPYVKA PDTHGNMWVP SHHHHHH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product TDP1 Protein
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- Target
- TDP1 (Tyrosyl-DNA Phosphodiesterase 1 (TDP1))
- Alternative Name
- TDP1 (TDP1 Products)
- Synonyms
- 2810481F14Rik Protein, 4921509N21Rik Protein, AI838772 Protein, AW493413 Protein, E430034L06Rik Protein, MLZ-501 Protein, SCAN1 Protein, tdp1 Protein, MGC83708 Protein, TDP1 Protein, F8M21.60 Protein, F8M21_60 Protein, tyrosyl-DNA phosphodiesterase 1 Protein, tyrosyl-DNA phosphodiesterase 1 Protein, tyrosyl-DNA Phosphodiesterase (Tdp1) Protein, tyrosyl-DNA phosphodiesterase 1 L homeolog Protein, tyrosyl-DNA phosphodiesterase-like protein Protein, TDP1 Protein, Tc00.1047053511191.10 Protein, Tdp1 Protein, tdp1.L Protein, tdp1 Protein, PTRG_11198 Protein, MCYG_02121 Protein, MGYG_03035 Protein
- Background
- TDP1, also known as tyrosyl-DNA phosphodiesterase 1, is involved in repairing stalled topoisomerase l-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase l and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical meditated DNA double-strand breaks. Recombinant human TDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 69.5kDa (617aa) 50-70kDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_060789
- UniProt
- Q9NUW8
-