Chromogranin A Protein (CHGA) (AA 19-457) (His tag)
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- Target See all Chromogranin A (CHGA) Proteins
- Chromogranin A (CHGA)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-457
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Chromogranin A protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADLLPVNSPM NKGDTEVMKC IVEVISDTLS KPSPMPVSQE CFETLRGDER ILSILRHQNL LKELQDLALQ GAKERAHQQK KHSGFEDELS EVLENQSSQA ELKEAVEEPS SKDVMEKRED SKEAEKSGEA TDGARPQALP EPMQESKAEG NNQAPGEEEE EEEEATNTHP PASLPSQKYP GPQAEGDSEG LSQGLVDREK GLSAEPGWQA KREEEEEEEE EAEAGEEAVP EEEGPTVVLN PHPSLGYKEI RKGESRSEAL AVDGAGKPGA EEAQDPEGKG EQEHSQQKEE EEEMAVVPQG LFRGGKSGEL EQEEERLSKE WEDSKRWSKM DQLAKELTAE KRLEGQEEEE DNRDSSMKLS FRARAYGFRG PGPQLRRGWR PSSREDSLEA GLPLQVRGYP EEKKEEEGSA NRRPEDQELE SLSAIEAELE KVAHQLQALR RGHHHHHH
- Purity
- > 85 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1ug of protein (determined by LAL method)
- Top Product
- Discover our top product CHGA Protein
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Chromogranin A (CHGA) (AA 165-435) protein (His tag)
CHGA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chromogranin A (CHGA) (AA 338-432) protein (GST tag)CHGA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Chromogranin A (CHGA) (AA 1-457) protein (GST tag)CHGA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 20 % glycerol, 0.1 mM PMSF
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Chromogranin A (CHGA)
- Alternative Name
- CHGA (CHGA Products)
- Synonyms
- CGA Protein, CG-ALPHA Protein, FSHA Protein, GPHA1 Protein, GPHa Protein, HCG Protein, LHA Protein, TSHA Protein, ChrA Protein, fj05f09 Protein, si:dkey-177p2.2 Protein, wu:fj05f09 Protein, zgc:101749 Protein, zgc:56075 Protein, CHGA Protein, cgA Protein, chga Protein, chromogranin A Protein, glycoprotein hormones, alpha polypeptide Protein, uncharacterized CHGA Protein, chromogranin A S homeolog Protein, CHGA Protein, CGA Protein, Chga Protein, chga Protein, chga.S Protein
- Background
- CHGA, also known as chromogranin-A isoform 1 preproprotein, is a member of neuroendocrine secretory proteins of the chromogranin / secretogranin family. It is a precursor of several functional peptides including vasostatin-1, vasostatin-2, pancreastatin, catestatin and parastatin. This peptide negatively regulates the neuroendocrine function of autocrine or nearby cells (paracrine). This protein induces and promotes the production of secretory granules containing insulin in pancreatic islet beta cells. Recombinant human CHGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 50kDa (448aa) 50-70kDa (SDS-PAGE under reducing conditions)
- NCBI Accession
- NP_001266
- UniProt
- P10645
- Pathways
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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