MECR Protein (AA 54-373) (His tag)
Quick Overview for MECR Protein (AA 54-373) (His tag) (ABIN667794)
Target
See all MECR ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 54-373
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Purification tag / Conjugate
- This MECR protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MPAKVVELKN LELAAVRGSD VRVKMLAAPI NPSDINMIQG NYGLLPELPA VGGNEGVAQV VAVGSNVTGL KPGDWVIPAN AGLGTWRTEA VFSEEALIQV PSDIPLQSAA TLGVNPCTAY RMLMDFEQLQ PGDSVIQNAS NSGVGQAVIQ IAAALGLRTI NVVRDRPDIQ KLSDRLKSLG AEHVITEEEL RRPEMKNFFK DMPQPRLALN CVGGKSSTEL LRQLARGGTM VTYGGMAKQP VVASVSLLIF KDLKLRGFWL SQWKKDHSPD QFKELILTLC DLIRRGQLTA PACSQVPLQD YQSALEASMK PFISSKQILT M
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Characteristics
- MECR, 54-373aa, Human, His tag, E.coli
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Purification
- > 90% by SDS-PAGE
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 5 mM DTT, 20 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- MECR (Mitochondrial Trans-2-Enoyl-CoA Reductase (MECR))
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Alternative Name
- MECR
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Background
- MECR (Mitochondrial trans-2-enoyl-CoA reductase), also known as NRBF1, catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH dependent manner with preference to medium chain length substrate. This protein has a role in the mitochondrial synthesis of fatty acids. Recombinant human MECR protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
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Molecular Weight
- 36.9 kDa (341aa) confirmed by MALDI-TOF
Target
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