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Leptin Protein (LEP)

This Leptin protein is expressed in Escherichia coli (E. coli).

Leptin Human Recombinant Protein - SDS-PAGE. (Leptin Protein (LEP))

1 image

Catalog No. ABIN6700211

Supplier Product No.: 009-001-320

Quick Overview for Leptin Protein (LEP) (ABIN6700211)

Target

See all Leptin (LEP) Proteins

Leptin (LEP)

Origin

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Human

Source

Escherichia coli (E. coli)

Discover our top product LEP Protein

Supplier Product No.

009-001-320

Supplier

Rockland

Cross-Reactivity

Human

Characteristics

Leptin, LEP cytokine, Obesity factor, Obese protein

Purification

Purity was determined to be greater than 95% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin Level

Low endotoxin
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Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Leptin (LEP) protein
LEP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by reducing SDS-PAGE.

Leptin (LEP) protein (His tag)
LEP Origin: Goldfish Host: Yeast Recombinant > 95 % as determined by reducing SDS-PAGE.

Leptin (LEP) (AA 22-167) protein (Fc Tag)
LEP Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Leptin (LEP) (AA 22-167) protein (His tag)
LEP Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA

Leptin (LEP) (AA 22-167) protein (His tag)
LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA

Leptin (LEP) (AA 428-635) protein (His tag)
LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA

Leptin (LEP) (AA 22-167) protein (GST tag)
LEP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Leptin (LEP) protein
LEP Origin: Rat Host: Escherichia coli (E. coli) Recombinant 95,00% SDS

Leptin (LEP) (AA 29-164), (partial) protein (GST tag)
LEP Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA

Application Notes

Application Note: Leptin is suitable as a control for polyclonal or monoclonal anti-Leptin in immunological assays.
Other Performance Data: Biological Activity: Human Recombinant Leptin is fully biologically active when compared to standard. The ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected mouse BaF/3 proliferation, is 1.36 ng/mL. Endotoxin Level: Measured by LAL is <0.01 ng/μg or <0.1EU/μg.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration

1.0 mg/mL

Buffer

Buffer: 0.1 % Trifluoroacetic acid

Preservative

Without preservative

Storage

RT,4 °C,-20 °C

Expiry Date

6 months
Target

Leptin (LEP)

Alternative Name

Leptin

UniProt

P41159

Pathways

JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process

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