Leptin Protein (LEP)

Catalog No. ABIN6700212

Supplier Product No.: 010-001-320

Leptin protein expressed in Escherichia coli (E. coli).

Quick Overview for Leptin Protein (LEP) (ABIN6700212)

Target: Leptin (LEP)

Origin: Mouse

Source: Escherichia coli (E. coli)

Product Details

Supplier Product No.: 010-001-320

Supplier: Rockland

Cross-Reactivity: Mouse (Murine)

Characteristics: Leptin, LEP cytokine, Obesity factor, Obese protein

Purification: Purity was determined to be greater than 95% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin Level: Low endotoxin

Application Details

Application Notes:

Application Note: Leptin is suitable as a control for polyclonal or monoclonal anti-Leptin in immunological assays.

Other Performance Data: Biological Activity: Mouse Recombinant Leptin is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent stimulation of Human OB-R transfected mouse BaF/3 proliferation, is 0.52 ng/mL. Endotoxin Level: Measured by LAL is <0.01 ng/μg or <0.1EU/μg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Expiry Date: 6 months

Target Details

Target: Leptin (LEP)

Alternative Name: Leptin

UniProt: P41160

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process