14-3-3 sigma/SFN Protein (GST tag)
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- Target See all 14-3-3 sigma/SFN (SFN) Proteins
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This 14-3-3 sigma/SFN protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Cross-Reactivity
- Human
- Characteristics
- 14-3-3 sigma, SFN, stratifin, YWHAS
- Purification
- Recombinant full-length human 14-3-3σ was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- Top Product
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Stratifin (SFN) (AA 1-248) protein (GST tag)
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Stratifin (SFN) (AA 1-248) protein (Strep Tag)Crystallography grade SFN Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Stratifin (SFN) (AA 1-248), (full length) protein (GST tag)SFN Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Stratifin (SFN) proteinSFN Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
Stratifin (SFN) protein (Myc-DYKDDDDK Tag)SFN Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Application Note: 14-3-3σ Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3σ Protein is suitable for use in Western Blot. Expect a band approximately ~ 51 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Storage
- -80 °C
- Storage Comment
- -80 °c
- Expiry Date
- 12 months
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- Target
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- Alternative Name
- 14-3-3 sigma (SFN Products)
- Synonyms
- YWHAS Protein, Er Protein, Mme1 Protein, Ywhas Protein, stratifin Protein, SFN Protein, Sfn Protein
- Pathways
- p53 Signaling, Myometrial Relaxation and Contraction
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