IL12RB1 Protein (AA 24-540) (His tag)

Catalog No. ABIN7275013

Product Details

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Protein Type: Recombinant

Protein Characteristics: AA 24-540

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12RB1 protein is labelled with His tag.

Purpose: Human IL-12 R beta 1/CD212 Protein

Sequence: Cys24-Glu540

Characteristics: Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys24-Glu540.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL12RB1 (Interleukin 12 Receptor beta 1 (IL12RB1))

Alternative Name: IL-12 R beta 1 (IL12RB1 Products)

Synonyms: Il12rb Protein, IL12RB1 Protein, IL-12R Protein, CD212 Protein, IL-12R-BETA1 Protein, IL12RB Protein, IL-12R[b] Protein, interleukin 12 receptor subunit beta 1 Protein, interleukin 12 receptor, beta 1 Protein, Il12rb1 Protein, IL12RB1 Protein

Background: Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).

Molecular Weight: 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation