CD70 Protein (AA 52-193) (His tag,AVI tag,DYKDDDDK Tag,Biotin)

Catalog No. ABIN6951035

Product Details

Target: CD70 (CD70 Molecule (CD70))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 52-193

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CD70 protein is labelled with His tag,AVI tag,DYKDDDDK Tag,Biotin.

Sequence: AA 52-193

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: CD70 (CD70 Molecule (CD70))

Alternative Name: CD27 Ligand (CD70 Products)

Synonyms: CD27LG Protein, Cd27l Protein, Tnfsf7 Protein, CD27L Protein, TNFSF7 Protein, CD70 antigen Protein, CD70 molecule Protein, Cd70 molecule Protein, Cd70 Protein, CD70 Protein

Background: Cluster of Differentiation 70 (CD70) is also known as CD27 ligand (CD27L / CD27LG), TNFSF7, TNFSF7G, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and is a surface antigen found on activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T-cells, and thus is invloved in the biological processes including T-cell activation, the proliferation of costimulated T-cells, as well as the generation of cytolytic T-cells. CD70 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation. CD70 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses. CD70 induces proliferation and IFNγ production, on NK cells.

Molecular Weight: 51.0 kDa

NCBI Accession: NP_001243

Pathways: Cancer Immune Checkpoints