SARS-CoV S1 Protein (His tag)

Catalog No. ABIN6952622

Product Details

Target: SARS-CoV S1 (SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1))

Protein Type: Recombinant

Origin: SARS Coronavirus (SARS-CoV)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV S1 protein is labelled with His tag.

Application: SDS-PAGE (SDS), ELISA

Purpose: SARS S1 protein, His Tag (MALS verified)

Sequence: AA 14-667

Characteristics: SARS S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Arg 667 (Accession # AAP13567.1). Predicted N-terminus: Ser 14
This protein carries a polyhistidine tag at the C-terminus.

Purity: >95 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.

References

Kondo, Iwatani, Matsuoka, Fujino, Umemoto, Yokomaku, Ishizaki, Kito, Sezaki, Hayashi, Murakami: "Antibody-like proteins that capture and neutralize SARS-CoV-2." in: Science advances, Vol. 6, Issue 42, (2020) (PubMed).

Tosif, Neeland, Sutton, Licciardi, Sarkar, Selva, Do, Donato, Quan Toh, Higgins, Van de Sandt, Lemke, Lee, Shoffner, Flanagan, Arnold, Mordant, Mulholland, Bines, Dohle, Pellicci, Curtis, McNab et al.: "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5703, (2020) (PubMed).

Target Details

Target: SARS-CoV S1 (SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1))

Alternative Name: SARS-Coronavirus Spike Subunit S1 (SARS-CoV S1 Products)

Target Type: Viral Protein

Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 74.9 kDa