TNFSF9 Protein (mFc-His Tag)

Catalog No. ABIN6961118

Product Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFSF9 protein is labelled with mFc-His Tag.

Purpose: Recombinant human 4-1BB Ligand Protein with N-terminal mouse Fc and C-terminal 6xHis tag

Specificity: MFc (Pro99-Lys330) 4-1BB Ligand (Pro52-Glu254) 6xHis

Characteristics: Extracellular Domain Protein

Purification: affinity purification

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with deionized water

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Preservative: Without preservative

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: 4-1BB Ligand (TNFSF9 Products)

Synonyms: TNFSF9 Protein, 4-1BB-L Protein, 4-1BBL Protein, AI848817 Protein, Cd137l Protein, Ly63l Protein, CD137L Protein, TNF superfamily member 9 Protein, tumor necrosis factor (ligand) superfamily, member 9 Protein, Tnfsf9 Protein, LOC476729 Protein, TNFSF9 Protein

Background: Synonymes: 4-1BB Ligand,TNFSF9,CD137L
Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Molecular Weight: predicted molecular mass of 49.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-4-1BB Ligand-His is 53-70 kDa due to glycosylation.

Gene ID: 8744

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints