TNFSF9 Protein (Trimer) (His tag)

Catalog No. ABIN7273928

Product Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Protein Type: Recombinant

Protein Characteristics: Trimer

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFSF9 protein is labelled with His tag.

Purpose: Human 4-1BB Ligand/TNFSF9 Trimer Protein

Sequence: Arg71-Glu254

Characteristics: Recombinant Human 4-1BB Ligand/TNFSF9 Trimer Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Arg71-Glu254.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human 4-1BB Ligand Trimer, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human 4-1BB, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: 4-1BB Ligand (TNFSF9 Products)

Synonyms: TNFSF9 Protein, 4-1BB-L Protein, 4-1BBL Protein, AI848817 Protein, Cd137l Protein, Ly63l Protein, CD137L Protein, TNF superfamily member 9 Protein, tumor necrosis factor (ligand) superfamily, member 9 Protein, Tnfsf9 Protein, LOC476729 Protein, TNFSF9 Protein

Background: The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.

Molecular Weight: 61.8 kDa same as Tris-Bis PAGE result.

UniProt: P41273

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints