AKT1 Protein (AA 122-443) (His tag)

Catalog No. ABIN7405406

Recombinant AKT1 protein expressed in Escherichia coli (E. coli).

Quick Overview for AKT1 Protein (AA 122-443) (His tag) (ABIN7405406)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), SDS-PAGE (SDS), Positive Control (PC), Immunogen (Imm)

Purity: > 97 %

Product Details

Protein Characteristics: AA 122-443

Purification tag / Conjugate: This AKT1 protein is labelled with His tag.

Sequence: Ser122-Thr443

Characteristics: Recombinant Protein Kinase B Alpha (PKBa), Prokaryotic expression, Ser122-Thr443, N-terminal His Tag

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Isoelectric Point:6.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Expiry Date: 12 months

Target Details

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: Protein Kinase B Alpha

Background: AKT1, AKT, PKB-A, Proto-oncogene c-Akt, RAC-Alpha Serine/Threonine-Protein Kinase, V-akt Murine Thymoma Viral Oncogene Homolog 1

Molecular Weight: 44kDa

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect