AKT1 Protein (His tag)

Catalog No. ABIN7198628

Product Details

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This AKT1 protein is labelled with His tag.

Purpose: Recombinant Human AKT1/PKB/PKBα Protein (His Tag)(Active)

Sequence: Met 1-Ala 480

Characteristics: A DNA sequence encoding the human AKT1 (NP_001014431.1) (Met 1-Ala 480) was fused with a polyhistidine tag at the C-terminus.

Purity: > 87 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1.No Kinase Activity 2. Measured by its ability to bind biotinylated human CD136 in a functional ELISA.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 3 mM DTT, 0.5 mM PMSF, 5 % Glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1/PKB/PKBalpha (AKT1 Products)

Synonyms: AKT Protein, CWS6 Protein, PKB Protein, PKB-ALPHA Protein, PRKBA Protein, RAC Protein, RAC-ALPHA Protein, Akt Protein, Rac Protein, PKB/Akt Protein, PKBalpha Protein, AKT/PKB Protein, AKT1 Protein, Akt/PKB Protein, Akt1 Protein, CG4006 Protein, D-Akt Protein, DAKT1 Protein, DAKT1/PKB Protein, DAkt Protein, DAkt1 Protein, DPKB Protein, DRAC-PK Protein, DRAC-PK66 Protein, DRAC-PK85 Protein, Dakt Protein, Dakt1 Protein, Dmel\\CG4006 Protein, Dpkb Protein, PKB/AKT Protein, PKB/dAKT Protein, RacPK Protein, akt Protein, akt1 Protein, dAKT Protein, dAKT/dPKB Protein, dAKT1 Protein, dAkt Protein, dAkt/PKB Protein, dAkt1 Protein, dPKB Protein, dakt Protein, dakt1 Protein, l(3)04226 Protein, l(3)89Bq Protein, p-Akt Protein, pAkt Protein, ACT-5 Protein, akt-1 Protein, pkb Protein, v-akt Protein, v-akt1 Protein, xAct Protein, ATAKT1 Protein, F18A8.2 Protein, F18A8_2 Protein, K+ transporter 1 Protein, POTASSIUM TRANSPORTER Protein, AKT serine/threonine kinase 1 Protein, thymoma viral proto-oncogene 1 Protein, CG4006 gene product from transcript CG4006-RE Protein, actin beta Protein, v-akt murine thymoma viral oncogene homolog 1 S homeolog Protein, v-akt murine thymoma viral oncogene homolog 1 Protein, K+ transporter 1 Protein, Serine/threonine-protein kinase akt-1 Protein, AKT1 Protein, Akt1 Protein, ACTB Protein, akt1.S Protein, akt1 Protein, KT1 Protein, akt-1 Protein

Background:

Background: v-akt murine thymoma viral oncogene homolog 1 (AKT1), or protein kinase B-alpha (PKB-ALPHA) is a serine-threonine protein kinase, belonging to the Protein Kinase Superfamily. AKT1 is a major mediator of the responses to insulin, insulin-like growth factor 1 (IGF1), and glucose. AKT1 also plays a key role in the regulation of both muscle cell hypertrophy and atrophy. AKT1 activity is required for physiologic cardiac growth in response to IGF1 stimulation or exercise training. In contrast, AKT1 activity was found to antagonize pathologic cardiac growth that occurs in response to endothelin 1 stimulation or pressure overload. AKT1 selectively promotes physiological cardiac growth while AKT2 selectively promotes insulin-stimulated cardiac glucose metabolism. AKT1 deletion prevented tumor initiation as well as tumor progression, coincident with decreased Akt signaling in tumor tissues. AKT1 is the primary Akt isoform activated by mutant K-ras in lung tumors, and that AKT3 may oppose AKT1 in lung tumorigenesis and lung tumor progression. A number of separate studies have implicated AKT1 as an inhibitor of breast epithelial cell motility and invasion. AKT1 may have a dual role in tumorigenesis, acting not only pro-oncogenically by suppressing apoptosis but also anti-oncogenically by suppressing invasion and metastasis.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA

Molecular Weight: 57 kDa

NCBI Accession: NP_001014431

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect