Claudin 18.2 (Active) protein (His-Avi Tag,Biotin)

Catalog No. ABIN7199041

Product Details

Target: Claudin 18.2

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: His-Avi Tag,Biotin

Purpose: Biotinylated Human Claudin-18.2 Protein, His,Avitag™ (active membrane protein)

Sequence: Met 1 - Ala 200

Characteristics: Biotinylated Human Claudin-18.2, His,Avitag (CL2-H82E3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Ala 200 (Accession # P56856-2).

Purity: >60 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24.8 kDa.
It is produced as active membrane protein with full ECD epitopes, and contains both the ECL1 and ECL2.

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH 7.5

Storage: -80 °C

Storage Comment: -70°C

Target Details

Target: Claudin 18.2

Alternative Name: Claudin-18.2

Background: Synonyms: Claudin-18.2,CLDN18,Claudin-18,
Description: Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.

Molecular Weight: 24.8 kDa

NCBI Accession: NP_001002026