GTPase NRas Protein (NRAS) (AA 1-186) (His tag)

Catalog No. ABIN7123231

Recombinant GTPase NRas protein expressed in Escherichia coli (E. coli).

Quick Overview for GTPase NRas Protein (NRAS) (AA 1-186) (His tag) (ABIN7123231)

Target: GTPase NRas (NRAS)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-186

Purification tag / Conjugate: This GTPase NRas protein is labelled with His tag.

Purpose: Recombinant Human NRAS

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background: ALPS4, AV095280, GTPase NRas, HRAS1, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, NRAS, NRAS1, NS6, OTTHUMP00000013879, OTTMUSP00000023521, RASN_HUMAN, Transforming protein N Ras, Transforming protein N-Ras, v ras neuroblastoma RAS viral oncogene homolog

Molecular Weight: 22.8 kDa

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling