GTPase NRas Protein (NRAS) (His tag)

Catalog No. ABIN7317421

This Recombinant GTPase NRas protein is produced in Escherichia coli (E. coli).

Quick Overview for GTPase NRas Protein (NRAS) (His tag) (ABIN7317421)

Target: GTPase NRas (NRAS)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This GTPase NRas protein is labelled with His tag.

Purpose: Recombinant Human NRAS Protein (His Tag)

Sequence: Met 1-Cys186

Characteristics: A DNA sequence encoding the mature form of human NRAS (P01111) (Met1-Cys186) was expressed with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Not validated for activity

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 0.1M NaCl, 10 % Glycerol, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: GTPase NRas (NRAS)

Alternative Name: NRAS

Background: ALPS4,CMNS,N-ras,NCMS,NRAS1,NS6,NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI).

Molecular Weight:

Calculated MW: 23 kDa

Observed MW: 24 kDa

Gene ID: 4893

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling