SIGLEC7 Protein (Glu201Ala-Mutant, Gly120Ala-Mutant, Leu118Ala-Mutant) (Fc Tag)
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- Target See all SIGLEC7 Proteins
- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
- Protein Type
- Recombinant
- Protein Characteristics
- Glu201Ala-Mutant, Gly120Ala-Mutant, Leu118Ala-Mutant
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SIGLEC7 protein is labelled with Fc Tag.
- Purpose
- Human Siglec-7 / CD328 Protein, Fc (L118A, G120A, E201A) Tag (MALS verified)
- Sequence
- Gln 19 - Leu 353
- Characteristics
- Human Siglec-7, Fc (L118A, G120A, E201A) Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).
- Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 0.01 EU per μg by the LAL method.
- Grade
- MALS verified
- Top Product
- Discover our top product SIGLEC7 Protein
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- Application Notes
- This protein carries a Fc (L118A, G120A, E201A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition due to glycosylation. Mutations (L118A, G120A, E201A /EU number: L235A/G237A/E318A) in human immunoglobulin G1 (hIgG1) Fc strongly reduce binding of the Fc mutant to cell expressed FcγRs, resulting in an almost 4-fold reduction in ADCC compared to that of wild type human IgG1.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 25 mM MES, 150 mM NaCl
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
- Alternative Name
- Siglec-7 / CD328 (SIGLEC7 Products)
- Synonyms
- SIGLEC7 Protein, AIRM1 Protein, CD328 Protein, CDw328 Protein, D-siglec Protein, QA79 Protein, SIGLEC-7 Protein, SIGLEC19P Protein, SIGLECP2 Protein, p75 Protein, p75/AIRM1 Protein, sialic acid-binding Ig-like lectin 7 Protein, sialic acid binding Ig like lectin 7 Protein, LOC468976 Protein, SIGLEC7 Protein
- Background
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Synonyms: CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1,
Siglec-7 is a member of the human CD33-related Siglec receptor. The extracellular region of Siglec-7 is characterized by an N-terminal V-set Ig domain that can bind sialic acid and two C2-set Ig domains. The cytoplasmic tail of Siglec-7 has one immune-receptor tyrosine-based inhibitory motif (ITIM) and one ITIM-like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells. - Molecular Weight
- 63.3 kDa
- NCBI Accession
- NP_055200
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