IL-2 R beta & IL-2 R alpha & IL-2 R gamma protein (His tag,Twin-Strep tag)

Catalog No. ABIN7125964

Product Details

Target: IL-2 R beta & IL-2 R alpha & IL-2 R gamma

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: His tag,Twin-Strep tag

Purpose: Human IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, His Tag&Twin Strep Tag (MALS verified)

Sequence: Ala 27 - Asp 239 (IL-2RB) & Glu 22 - Ser 212 (IL-2RA) & Leu 23 - Asn 254 (IL-2RG)

Characteristics: Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Glu 22 - Ser 212 (IL-2RA) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P01589-1 (IL-2RA) & P31785-1 (IL-2RG).

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Grade: MALS verified

Application Details

Application Notes: Human IL-2RB&IL-2RA&IL-2RG, His Tag&Twin Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 29.9 kDa (IL-2RB) & 55.8 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 37 kDa, 42 kDa and 90 kDa respectively due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: IL-2 R beta & IL-2 R alpha & IL-2 R gamma

Alternative Name: IL-2 R beta&IL-2 R alpha&IL-2 R gamma

Background: Synonyms: IL-2 R beta & IL-2 R alpha & IL-2 R gamma,
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Molecular Weight: 29.9 kDa (IL-2RB) & 55.8 kDa (IL-2RA&IL-2RG)