SIGLEC7 Protein (Leu117Ala-Mutant, Leu118Ala-Mutant) (Fc Tag)

Catalog No. ABIN7125985

Product Details

Target: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Protein Type: Recombinant

Protein Characteristics: Leu117Ala-Mutant, Leu118Ala-Mutant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SIGLEC7 protein is labelled with Fc Tag.

Purpose: Human Siglec-7 / CD328 Protein, Fc (L117A, L118A) Tag (MALS verified)

Sequence: Gln 19 - Leu 353

Characteristics: Human Siglec-7, Fc (L117A, L118A) Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 0.01 EU per μg by the LAL method.

Grade: MALS verified

Application Details

Application Notes: This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 25 mM MES, 150 mM NaCl, pH 5.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Alternative Name: Siglec-7 / CD328 (SIGLEC7 Products)

Synonyms: SIGLEC7 Protein, AIRM1 Protein, CD328 Protein, CDw328 Protein, D-siglec Protein, QA79 Protein, SIGLEC-7 Protein, SIGLEC19P Protein, SIGLECP2 Protein, p75 Protein, p75/AIRM1 Protein, sialic acid-binding Ig-like lectin 7 Protein, sialic acid binding Ig like lectin 7 Protein, LOC468976 Protein, SIGLEC7 Protein

Background: Synonyms: CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1,
Siglec-7 is a member of the human CD33-related Siglec receptor. The extracellular region of Siglec-7 is characterized by an N-terminal V-set Ig domain that can bind sialic acid and two C2-set Ig domains. The cytoplasmic tail of Siglec-7 has one immune-receptor tyrosine-based inhibitory motif (ITIM) and one ITIM-like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.

Molecular Weight: 63.3 kDa

NCBI Accession: NP_055200