LGMN Protein (Cys1, full length) (rho-1D4 tag)

Catalog No. ABIN7126042

Product Details

Target: LGMN (Legumain (LGMN))

Protein Type: Recombinant

Protein Characteristics: Cys1, full length

Origin: CHO cells

Source: Insect Cells

Purification tag / Conjugate: This LGMN protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequence: MCRMIWKAAV LLSLALGAGA LAVGVDDPED AGKHWVVIVA GSNGWYNYRH QADACHAYQI IHRNGIPDEQ IIVMMYDDIA NSEDNPTPGI VINRPNGTDV YAGVLKDYTG EDVTPENFLA VLRGDAEAVK GKGSGKVLRS GPQDHVFVYF TDHGATGLLV FPNEDLHVKD LNKTIRYMYE HKMYQKMVFY IEACESGSMM NHLPNDINVY ATTAANPHES SYACYYDEER NTYLGDWYSV NWMEDSDVED LTKETLHKQY HLVKSHTNTS HVMQYGNKSI STMKVMQFQG MKHSTSSPIS LPPVTRLDLT PSPEVPLTIL KRKLMSTNDL KQSQNLVGQI QRLLDARHVI EKSVHKIVSL LAGFGETAER LLSERAVLMA HDCYQEAVTH FRTHCFNWHS PTYEYALRHL YVLANLCEKP YPIDRIKMAM DKVCLSHY
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Legumain (Asparaginyl endopeptidase) (Protease, cysteine 1) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Grade: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LGMN (Legumain (LGMN))

Alternative Name: Legumain (Asparaginyl endopeptidase) (Protease) (LGMN Products)

Synonyms: AEP Protein, LGMN1 Protein, PRSC1 Protein, AI746452 Protein, AU022324 Protein, Prsc1 Protein, MGC64351 Protein, cb173 Protein, cb460 Protein, sb:cb173 Protein, zgc:76953 Protein, wu:fa98g05 Protein, wu:fj92g08 Protein, lgmn Protein, MGC88980 Protein, DELTA VACUOLAR PROCESSING ENZYME Protein, DELTAVPE Protein, delta vacuolar processing enzyme Protein, legumain Protein, legumain L homeolog Protein, delta vacuolar processing enzyme Protein, Legumain Protein, LGMN Protein, Lgmn Protein, lgmn.L Protein, lgmn Protein, DELTA-VPE Protein, MBOO_RS07785 Protein, Odosp_3582 Protein, LOC569153 Protein

UniProt: G3I1H5

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades