Histone H2B Protein (full length) (rho-1D4 tag)
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- Target See all Histone H2B Proteins
- Histone H2B
- Protein Type
- Recombinant
- Protein Characteristics
- full length
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Origin
- CHO cells
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Source
- Insect Cells
- Purification tag / Conjugate
- This Histone H2B protein is labelled with rho-1D4 tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)
- Sequence
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MPEPSKSAPA PKKGSKKAIT KAQKKDGKKR KRSRKESYSV YVYKVLKQVH PDTGISSKAM GIMNSFVNDI FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSSK
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Histone H2B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- Grade
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product Histone H2B Protein
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Histone H2B (AA 1-126) protein (GST tag)
Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Histone H2B
- Abstract
- Histone H2B Products
- Synonyms
- Hist1h2bc Protein, H2B Protein, HISTONE H2B Protein, MRN17.11 Protein, MRN17_11 Protein, histone B2 Protein, H2B1 Protein, HIS2B Protein, histone cluster 1 H2B family member k Protein, histone H2B Protein, histone B2 Protein, histone H2B.3 Protein, Hist1h2bk Protein, H2B Protein, HTB2 Protein, THAPSDRAFT_27352 Protein, THAPSDRAFT_37309 Protein, THAPS_6947 Protein, THAPSDRAFT_34210 Protein, LOC426037 Protein, LOC107802329 Protein
- UniProt
- G3HDU3
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