MERTK Protein (GST tag,His tag)

Catalog No. ABIN7320009

Product Details

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MERTK protein is labelled with GST tag,His tag.

Purpose: Recombinant Mouse MERTK/MER Protein (His & GST Tag)(Active)

Sequence: Glu573-Tyr867

Characteristics: A DNA sequence encoding the mouse MERTK (Q60805) (Glu573-Tyr867) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 96 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 30 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MERTK (C-Mer Proto-Oncogene Tyrosine Kinase (MERTK))

Alternative Name: MERTK/MER (MERTK Products)

Synonyms: MER Protein, RP38 Protein, c-mer Protein, Eyk Protein, Mer Protein, Nyk Protein, nmf12 Protein, rdy Protein, C-EYK Protein, MERTK Protein, DKFZp469D2215 Protein, si:ch1073-268l12.2 Protein, MER proto-oncogene, tyrosine kinase Protein, c-mer proto-oncogene tyrosine kinase Protein, c-mer proto-oncogene tyrosine kinase a Protein, MERTK Protein, Mertk Protein, mertka Protein

Background:

Background: Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.

Synonym: Eyk,Mer,nmf12,Nyk

Molecular Weight: 61.7 kDa

UniProt: Q60805

Pathways: RTK Signaling