Elastase 4 Protein (His tag)

Catalog No. ABIN7195125

Product Details

Target: Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Elastase 4 protein is labelled with His tag.

Purpose: Recombinant Human Chymotrypsin C Protein (His Tag)(Active)

Sequence: Met 1-Leu 268

Characteristics: A DNA sequence encoding the human CTRC (Q99895) (Met 1-Leu 268) was fused with a polyhistidine tag at the C-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC.The specific activity is >300 pmol/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Elastase 4 (CTRC) (Chymotrypsin C (Caldecrin) (CTRC))

Alternative Name: Chymotrypsin C (CTRC Products)

Synonyms: CLCR Protein, ELA4 Protein, 1810044E12Rik Protein, bPTLP Protein, chymotrypsin C Protein, chymotrypsin-C Protein, chymotrypsin C (caldecrin) Protein, CTRC Protein, LOC478220 Protein, Ctrc Protein

Background:

Background: Chymotrypsin C (abbreviated for CTRC); also known as caldecrin or elastase4; is a digestive enzyme of the peptidase S1 family. This enzyme is synthesized as an inactivate chymotrypsinogen. On cleavage by trypsin into two parts that activate each other by removing two small peptides in a trans-proteolysis; chymotrypsin C produced. N-linked glycosylation of human CTRC is required for efficient folding and secretion; however; the N-linked glycan is unimportant for enzyme activity or inhibitor binding. It has been proposed that CTRC is a key regulator of digestive zymogen activation and a physiological co-activator of digestive carboxypeptidases proCPA1 and proCPA2. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis. It's speculated that CTRC might regulate pancreatic cancer cell migration in relation to cytokeratin 18 expression. The pancreatic cancer cell migration ability was downregulated in pancreatic cancer Aspc-1 cells that overexpressed CTRC; whereas the cell migration ability was upregulated in Aspc-1 cells in which CTRC was suppressed. 

Synonym: Chymotrypsin-C; Caldecrin; CTRC; CLCR

Molecular Weight: 29.3 kDa

UniProt: Q99895