COL2A1 Protein (AA 1242-1487) (His tag)

Catalog No. ABIN7317486

Product Details

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Protein Type: Recombinant

Protein Characteristics: AA 1242-1487

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This COL2A1 protein is labelled with His tag.

Purpose: Recombinant Human COL2A1 Protein (aa 1242-1487, His Tag)

Sequence: Asp 1242-Leu 1487

Characteristics: A DNA sequence encoding the amino acids (Asp 1242-Leu 1487) of human COL2A1 isoform 1 (P02458-2), known as chondrocalcin, was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Alternative Name: COL2A1 (COL2A1 Products)

Synonyms: ANFH Protein, AOM Protein, COL11A3 Protein, SEDC Protein, Col2 Protein, Col2a Protein, Col2a-1 Protein, Del1 Protein, Dmm Protein, Lpk Protein, M100856 Protein, Rgsc856 Protein, CG2A1A Protein, COLLII Protein, col2a1a Protein, CB11 Protein, col2a1 Protein, coll2a1 Protein, fb38c06 Protein, fc10c01 Protein, wu:fb38c06 Protein, wu:fc10c01 Protein, collagen type II alpha 1 chain Protein, collagen, type II, alpha 1 Protein, collagen, type II, alpha 1 L homeolog Protein, collagen, type II, alpha 1 S homeolog Protein, collagen, type II, alpha 1a Protein, COL2A1 Protein, Col2a1 Protein, col2a1 Protein, col2a1.L Protein, col2a1.S Protein, col2a1a Protein

Background:

Background: COL2A1 is the alpha-1 chain of type II collagen which is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this protein are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. Type II collagen is specific for cartilaginous tissues. Thus COL2A1 is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. The regulation of COL2A1, likely results from a balance of both positive and negative proteins. The inhibition of COL2A1 transcription following treatment of chick sternal chondrocytes with growth factors was accompanied by increased EF1 expression. Overexpression of EF1 in differentiated chondrocytes resulted in decreased expression of a reporter construct containing a collagen II promoter/enhancer insert.

Synonym: ANFH,AOM,COL11A3,SEDC,STL1

Molecular Weight: 29.8 kDa

Pathways: Sensory Perception of Sound, Growth Factor Binding