CNTN3 Protein (Fc Tag)

Catalog No. ABIN7317990

Product Details

Target: CNTN3 (Contactin 3 (Plasmacytoma Associated) (CNTN3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This CNTN3 protein is labelled with Fc Tag.

Purpose: Recombinant Human Contactin 3/CNTN3 Protein (708 Asp/Asn, Fc Tag)(Active)

Sequence: Met 1-Ser 1002, 708 Asp/Asn

Characteristics: A DNA sequence encoding the human CNTN3 precursor (NP_065923.1) (Met 1-Ser 1002, 708 Asp/Asn) was expressed with the fused Fc region of human IgG1 at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CNTN3 coated plates (0.8 μg/ml and 100 μl/well), approximately 30%-50% will adhere specifically after 60 minutes at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: CNTN3 (Contactin 3 (Plasmacytoma Associated) (CNTN3))

Alternative Name: Contactin 3/CNTN3 (CNTN3 Products)

Synonyms: Pang Protein, BIG-1 Protein, PANG Protein, PCS Protein, contactin 3 Protein, CNTN3 Protein, Cntn3 Protein

Background:

Background: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2(TAG-1), Contactin-3(BIG-1), BIG-2, Contactin-5(NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-3, also known as CNTN3 ( BIG-1 in rat and PANG in mouse ), is a GPI-linked glycoprotein that is expressed on cerebellar Purkinje cells, amygdaloid and thalamic neurons and olfactory granule cells. In the brain, Contactin-3 is expressed in frontal lobe, occipital lobe, cerebellum and amygdala. Contactin-3 contains 4 fibronectin type-III domains and 6 Ig-like C2-type (immunoglobulin-like) domains. Human Contactin-3 shares 92 % aa identity with mouse Contactin-3.The exact function of Contactin-3 is unclear. Contactin-3 may mediate cell-cell interaction and may promote neurite outgrowth.

Synonym: BIG-1,PANG,PCS

Molecular Weight: 134.5 kDa

NCBI Accession: NP_065923