Cyclin H Protein (CCNH) (His tag)

Catalog No. ABIN7318366

Product Details

Target: Cyclin H (CCNH)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Cyclin H protein is labelled with His tag.

Purpose: Recombinant Human Cyclin-H/CCNH Protein (His Tag)

Sequence: Met 1-Leu323

Characteristics: Recombinant Human Cyclin-H is produced by our E.coli expression system and the target gene encoding Met1-Leu323 is expressed with a 6His tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,100 mM NaCl,2 mM EDTA,2 mM DTT,30 % Glycerol, pH 8.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: Cyclin H (CCNH)

Alternative Name: Cyclin-H/CCNH (CCNH Products)

Synonyms: zgc:114132 Protein, cak Protein, p34 Protein, p37 Protein, Cych Protein, ACYPI004637 Protein, ccnh Protein, Cyclin-H Protein, 142767_at Protein, CG7405 Protein, Dmel\\CG7405 Protein, CAK Protein, 6330408H09Rik Protein, AI661354 Protein, AV102684 Protein, AW538719 Protein, ccnh-A Protein, cyclin H Protein, cyclin-H Protein, Cyclin H Protein, CYclin H Protein, cyclin H L homeolog Protein, CCNH Protein, ccnh Protein, LOC5577539 Protein, Cych Protein, CycH Protein, cyh-1 Protein, Ccnh Protein, ccnh.L Protein

Background:

Background: Human CCNH, also known as Cyclin-H,MO15-associated protein,p34 and p37, is a protein which belongs to the highly conserved cyclin family. Cyclin family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1.CCNH regulates CDK7 which is the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. CCNH is also involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Synonym: Cyclin-H,CCNH,MO15-associated protein,p34,p37

Molecular Weight: 39.8 kDa

UniProt: P51946

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase