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FH Protein (His tag)

FH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Catalog No. ABIN7317412
  • Target See all FH Proteins
    FH (Fumarate Hydratase (FH))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 7
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 7
    • 3
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This FH protein is labelled with His tag.
    Purpose
    Recombinant Human Fumarase/FH Protein (His Tag)(Active)
    Sequence
    Ala 45-Lys 510
    Characteristics
    A DNA sequence encoding the mature form of human FH (P07954-1) (Ala 45-Lys 510) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
    Purity
    > 85 % as determined by reducing SDS-PAGE.
    Biological Activity Comment
    Measured by its ability to transform 1umole of Fumarate to L-malate per minute at pH 7.5 at 37!aC. Specific activity is > 25 unit/mg
    Top Product
    Discover our top product FH Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Please refer to the printed manual for detailed information.
    Buffer
    Lyophilized from sterile 10 mM Tris, 5 mM EDTA, 1 mM DTT, pH 7.5
    Storage
    4 °C,-20 °C,-80 °C
    Storage Comment
    Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
  • Target
    FH (Fumarate Hydratase (FH))
    Alternative Name
    Fumarase/FH (FH Products)
    Synonyms
    FH Protein, DDBDRAFT_0205237 Protein, DDBDRAFT_0231397 Protein, DDBDRAFT_0231400 Protein, DDB_0205237 Protein, DDB_0231397 Protein, DDB_0231400 Protein, HLRCC Protein, LRCC Protein, MCL Protein, MCUL1 Protein, Fh1 Protein, im:7152785 Protein, ns:zf-e152 Protein, zf-e152 Protein, zgc:66253 Protein, zgc:77498 Protein, Fh Protein, Fh-1 Protein, fumarate hydratase Protein, fumarate hydratase 1 Protein, FH Protein, fumH Protein, Fh Protein, fh Protein, Fh1 Protein
    Background

    Background: Fumarate Hydratase (FH) is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. Fumarate Hydratase is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Individuals with hemizygous germline fumarate hydratase (FH) mutations are predisposed to renal cancer. These tumors predominantly exhibit functional inactivation of the remaining wild-type allele, implicating FH inactivation as a tumor-promoting event.

    Synonym: Fumarate Hydratase Mitochondrial, Fumarase, FH,FMRD,HLRCC,LRCC,MCL,MCUL1

    Molecular Weight
    52 kDa
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