LGALS8 Protein (GST tag)

Catalog No. ABIN7317925

Product Details

Target: LGALS8 (Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LGALS8 protein is labelled with GST tag.

Purpose: Recombinant Human Galectin-8/LGALS8 Protein (GST Tag)(Active)

Sequence: Met 1-Trp 317

Characteristics: A DNA sequence encoding human LGALS8 (AAF19370.1) (Met 1-Trp 317) was fused with the GST tag at the N-terminus.

Purity: > 76 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.2-1 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 0.1 M NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: LGALS8 (Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))

Alternative Name: Galectin-8/LGALS8 (LGALS8 Products)

Synonyms: LGALS8 Protein, 1200015E08Rik Protein, AI326142 Protein, D13Ertd524e Protein, Lgals-8 Protein, galectin-8 Protein, Gal-8 Protein, PCTA-1 Protein, PCTA1 Protein, Po66-CBP Protein, xgalectin-VIIIa Protein, galectin 8 Protein, lectin, galactose binding, soluble 8 Protein, lectin, galactoside binding soluble 8 S homeolog Protein, LGALS8 Protein, Lgals8 Protein, lgals8.S Protein

Background:

Background: Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state in order to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumour growth, has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumour progression including cell adhesion, migration and tumour-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host-pathogen interactions.

Synonym: Galectin-8, Gal-8, Po66 Carbohydrate-Binding Protein, Po66-CBP, Prostate Carcinoma Tumor Antigen 1, PCTA-1, LGALS8

Molecular Weight: 62.7 kDa