ERK2 Protein (GST tag)

Catalog No. ABIN7318068

Product Details

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This ERK2 protein is labelled with GST tag.

Purpose: Recombinant Human ERK2/MAPK1/MAPK2 Protein (GST Tag)

Sequence: Met 1-Ser 360

Characteristics: A DNA sequence encoding the human ERK2 (NP_002736.3) (Met 1-Ser 360) was fused with the GST tag at the N-terminus.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10 % Glycerol, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: ERK2/MAPK1/MAPK2 (MAPK1 Products)

Synonyms: ERK-1 Protein, ERK1 Protein, ERT2 Protein, HS44KDAP Protein, HUMKER1A Protein, P44ERK1 Protein, P44MAPK Protein, PRKM3 Protein, p44-ERK1 Protein, p44-MAPK Protein, Erk-1 Protein, Erk1 Protein, Ert2 Protein, Esrk1 Protein, Mnk1 Protein, Mtap2k Protein, Prkm3 Protein, p44 Protein, p44erk1 Protein, p44mapk Protein, ERK Protein, ERK2 Protein, ERT1 Protein, MAPK2 Protein, P42MAPK Protein, PRKM1 Protein, PRKM2 Protein, p38 Protein, p40 Protein, p41 Protein, p41mapk Protein, 9030612K14Rik Protein, AA407128 Protein, AU018647 Protein, C78273 Protein, Erk2 Protein, Prkm1 Protein, p42mapk Protein, zERK2 Protein, MAPK1 Protein, Xp42 Protein, erk Protein, erk2 Protein, ert1 Protein, mapk Protein, mapk1b Protein, mapk2 Protein, mpk1 Protein, prkm1 Protein, prkm2 Protein, mapk1 Protein, mapk1a Protein, xp42 Protein, ATMPK1 Protein, F14N23.9 Protein, F14N23_9 Protein, MITOGEN-ACTIVATED PROTEIN KINASE Protein, mitogen-activated protein kinase 1 Protein, MNK1 Protein, ATMPK2 Protein, MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2 Protein, T30E16.13 Protein, T30E16_13 Protein, mitogen-activated protein kinase homolog 2 Protein, mitogen-activated protein kinase 3 Protein, mitogen-activated protein kinase 1 Protein, mitogen activated protein kinase 1 Protein, mitogen-activated protein kinase 1 L homeolog Protein, mitogen-activated protein kinase 1 S homeolog Protein, mitogen activated protein kinase 3 Protein, mitogen-activated protein kinase homolog 2 Protein, MAPK3 Protein, Mapk3 Protein, MAPK1 Protein, Mapk1 Protein, mapk1 Protein, mapk1.L Protein, mapk1.S Protein, PfMAP1 Protein, MPK1 Protein, PVX_084965 Protein, LOC9328617 Protein, Tsp_01601 Protein, MPK2 Protein

Background:

Background: MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. ERK is a versatile protein kinase that regulates many cellular functions. Growing evidence suggests that extracellular signal-regulated protein kinase 1/2 (ERK1/2) plays a crucial role in promoting cell death in a variety of neuronal systems, including neurodegenerative diseases. It is believed that the magnitude and the duration of ERK1/2 activity determine its cellular function. Activation of ERK1/2 are implicated in the pathophysiology of spinal cord injury (SCI). ERK2 signaling is a novel target associated with the deleterious consequences of spinal injury. ERK-2, also known as Mitogen-activated protein kinase 1 (MAPK1), is a member of the protein kinase superfamily and MAP kinase subfamily. MKP-3 is a dual specificity phosphatase exclusively specific to MAPK1 for its substrate recognition and dephosphorylating activity. The activation of MAPK1 requires its phosphorylation by upstream kinases. Upon activation, MAPK1 translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. MAPK1 is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. MAPK1 acts as a transcriptional repressor which represses the expression of interferon gamma-induced genes. Transcriptional activity is independent of kinase activity. The nuclear-cytoplasmic distribution of ERK2 is regulated in response to various stimuli and changes in cell context. Furthermore, the nuclear flux of ERK2 occurs by several energy- and carrier-dependent and -independent mechanisms. ERK2 has been shown to translocate into and out of the nucleus by facilitated diffusion through the nuclear pore, interacting directly with proteins within the nuclear pore complex, as well as by karyopherin-mediated transport. ERK2 interacts with the PDE4 catalytic unit by binding to a KIM (kinase interaction motif) docking site located on an exposed beta-hairpin loop and an FQF (Phe-Gln-Phe) specificity site located on an exposed alpha-helix. These flank a site that allows phosphorylation by ERK, the functional outcome of which is orchestrated by the N-terminal UCR1/2 (upstream conserved region 1 and 2) modules.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: ERK;ERK-2;ERK2;ERT1;MAPK2;p38;p40;p41;p41mapk;p42-MAPK;P42MAPK;PRKM1;PRKM2

Molecular Weight: 67 kDa

NCBI Accession: NP_002736

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins