MUSK Protein (AA 433-783) (GST tag,His tag)

Catalog No. ABIN7317059

Product Details

Target: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Protein Type: Recombinant

Protein Characteristics: AA 433-783

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MUSK protein is labelled with GST tag,His tag.

Purpose: Recombinant Human MUSK Kinase Protein (aa 433-783, His & GST Tag)

Sequence: Arg 433-Val 783

Characteristics: A DNA sequence encoding the C-terminal segment of human MUSK isoform 2 (O15146-2) (Arg 433-Val 783) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 mM GSH.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Alternative Name: MUSK Kinase (MUSK Products)

Synonyms: unp Protein, fj98e12 Protein, zgc:63467 Protein, wu:fj98e12 Protein, si:dz46m7.2 Protein, si:dz57f23.3 Protein, si:busm1-57f23.3 Protein, musk-A Protein, MuSK Protein, MUSK Protein, Mdk4 Protein, Mlk Protein, Nsk1 Protein, Nsk2 Protein, Nsk3 Protein, muscle Protein, muscle associated receptor tyrosine kinase Protein, muscle, skeletal, receptor tyrosine kinase Protein, muscle associated receptor tyrosine kinase S homeolog Protein, MUSK Protein, musk Protein, musk.S Protein, Musk Protein

Background:

Background: Muscle, skeletal receptor tyrosine-protein kinase, also known as Muscle-specific tyrosine-protein kinase receptor, Muscle-specific kinase receptor, and MUSK, is a single-pass type I membrane protein which belongs to the protein kinase superfamily and tyr protein kinase family. MUSK contains one FZ (frizzled) domain, three Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. This protein is a muscle-specific tyrosine kinase receptor and it may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. MUSK expression is increased in muscle cells stimulated with Wnt or at conditions when the Wnt signaling was activated. MUSK is a muscle-specific receptor tyrosine kinase that is activated by agrin. It has a critical role in neuromuscular synapse formation. MUSK is a receptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization. Defects in MUSK encoding gene is a cause of autosomal recessive congenital myasthenic syndrome (CMS). Congenital myasthenic syndromes are inherited disorders of neuromuscular transmission that stem from mutations in presynaptic, synaptic, or postsynaptic proteins. MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction. Mutations in this receptor encoding gene also have been associated with congenital myasthenic syndrome.

Synonym: CMS9,FADS

Molecular Weight: 68 kDa

Pathways: RTK Signaling, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development