NCR3 Protein (His tag,Fc Tag)

Catalog No. ABIN7197099

Product Details

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NCR3 protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Human NCR3/NKp30 Protein (His & Fc Tag)(Active)

Sequence: Met 1-Gly 135

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Gly 135) of human NCR3 (NP_667341.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human B7-H6 -His at 10μg/mL (100μL/well) can bind human NCR3-Fch, the EC50 of human NCR3-Fch is 6-200ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Alternative Name: NCR3/NKp30 (NCR3 Products)

Synonyms: MGC133750 Protein, 1C7 Protein, CD337 Protein, LY117 Protein, MALS Protein, NKp30 Protein, Nkp30 Protein, natural cytotoxicity triggering receptor 3 Protein, NCR3 Protein, Ncr3 Protein

Background:

Background: Natural Cytotoxicity Triggering Receptor 3, NCR3, also known as NKp30, or CD337, is a natural cytotoxicity receptor, expressed on subsets of human peripheral blood NK cells, involved in NK cell killing of tumor cells and immature dendritic cells. The cellular ligand for NKp30 has remained elusive, but the membrane-associated heparan sulfate (HS) proteoglycans are involved in the recognition of cellular targets by NKp30 was recently reported. NKp30 is a member of the immunoglobulin superfamily and one of three existing natural cytotoxicity-triggering receptors. NKp30 is a glycosylated protein and is thought to be selectively expressed in resting and activated natural killer cells. NKp30 is a stimulatory receptor on human NK cells implicated in tumor immunity, and is capable of promoting or terminating dendritic cell maturation. NCR3 may play a role in inflammatory and infectious diseases.

Synonym: Natural Cytotoxicity Triggering Receptor 3, Activating Natural Killer Receptor p30, Natural Killer Cell p30-Related Protein, NK-p30, NKp30, CD337, NCR3, 1C7, LY117,1C7,DAAP-90L16.3,MALS

Molecular Weight: 41 kDa

NCBI Accession: NP_667341

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process