NCF1 Protein (His tag)
-
- Target See all NCF1 Proteins
- NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NCF1 protein is labelled with His tag.
- Purpose
- Recombinant Human Neutrophil Cytosol Factor 1/NCF1 Protein (His Tag)
- Sequence
- Met 1-Val390
- Characteristics
- Recombinant Human Neutrophil cytosol factor 1 is produced by our E.coli expression system and the target gene encoding Met1-Val390 is expressed with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product NCF1 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris 100 mM NaCl,1 mM DTT, pH 8.0.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- NCF1 (Neutrophil Cytosol Factor 1 (NCF1))
- Alternative Name
- Neutrophil Cytosol Factor 1/NCF1 (NCF1 Products)
- Synonyms
-
NCF1A Protein, NOXO2 Protein, SH3PXD1A Protein, p47phox Protein, Ncf-1 Protein, p47
Protein, neutrophil cytosolic factor 1 Protein, NCF1 Protein, Ncf1 Protein - Background
-
Background: Neutrophil cytosol factor 1( NCF1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is characterized as a multicomponent enzyme which is activated to produce superoxide anion. NCF2, NCF1, and a membrane bound cytochrome b558 are required for the activation of the latent NADPH oxidase. The human NCF1 gene encodes a 390 amino acids protein without a signal peptide. The NCF1 gene interacts with other subunits of nicotinamide adenine dinucleotide phosphate-oxidase (NADPH) and plays an important role in innate immunity, producing reactive oxygen species and reducing the severity and duration of parasitic infection and autoimmune disease. NCF1 also has a role in T cell activation.
Synonym: NCF-1,47 kDa autosomal chronic granulomatous disease protein/47 kDa neutrophil oxidase factor,NCF-47K,Neutrophil NADPH oxidase factor 1,Nox organizer 2,Nox-organizing protein 2/SH3 and PX domain-containing protein 1A,p47-phox
- Molecular Weight
- 45.6 kDa
- UniProt
- P14598
- Pathways
- PI3K-Akt Signaling
-