PDE4B Protein (GST tag,His tag)

Catalog No. ABIN7317546

Product Details

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PDE4B protein is labelled with GST tag,His tag.

Purpose: Recombinant Human PDE4B/DPDE4 Protein (His & GST Tag)

Sequence: Met 1-Thr 564

Characteristics: A DNA sequence encoding the human PDE4B isofrom 2 (NP_001032416.1) (Met 1-Thr 564) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 10 % glycerol, 0.5 mM PMSF, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))

Alternative Name: PDE4B/DPDE4 (PDE4B Products)

Synonyms: dpde4 Protein, pde4b5 Protein, pdeivb Protein, Dpde4 Protein, R74983 Protein, dunce Protein, PDE4/IVb Protein, Pde4B1 Protein, Pde4B2 Protein, Pde4B3 Protein, Pde4B4 Protein, DPDE4 Protein, PDE4B5 Protein, PDEIVB Protein, phosphodiesterase 4B Protein, phosphodiesterase 4B, cAMP specific Protein, phosphodiesterase 4B S homeolog Protein, pde4b Protein, PDE4B Protein, Pde4b Protein, pde4b.S Protein

Background:

Background: cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B / DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B / DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents

Synonym: DPDE4;PDEIVB

Molecular Weight: 92.2 kDa

NCBI Accession: NP_001032416

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction