Poliovirus Receptor Protein (PVR) (AA 29-348) (His tag)

Catalog No. ABIN7320731

Product Details

Target: Poliovirus Receptor (PVR)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 29-348

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This Poliovirus Receptor protein is labelled with His tag.

Purpose: Recombinant Mouse CD155/PVR Protein (aa 29-348, His Tag)(Active)

Sequence: Asp29-Leu348

Characteristics: Recombinant Mouse Poliovirus Receptor is produced by our Mammalian expression system and the target gene encoding Asp29-Leu348 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human TIGIT-Fc(Cat: PKSH033510) at 5μg/ml(100 μl/well) can bind Mouse PVR-6His. The ED50 of Mouse PVR-6His is 35.3 ug/mL

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Poliovirus Receptor (PVR)

Alternative Name: CD155/PVR (PVR Products)

Synonyms: PVR Protein, CD155 Protein, HVED Protein, NECL5 Protein, Necl-5 Protein, PVS Protein, TAGE4 Protein, 3830421F03Rik Protein, D7Ertd458e Protein, Taa1 Protein, Tage4 Protein, mE4 Protein, necl-5 Protein, poliovirus receptor Protein, PVR Protein, Pvr Protein

Background:

Background: Mouse poliovirus receptor (PVR, CD155) is a type I transmembrane (TM) glycoprotein that is a member of the nectin-related family of adhesion proteins within the immunoglobulin superfamily. It binds other molecules including vitronectin, Nectin3, DNAM1, CD96, and TIGIT, but does not bind homotypically. CD155 includes a 28 aa signal sequence, a 318 aa extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa TM segment and a 38 aa cytoplasmic tail. Epithelial, endothelial, and many immune cells show low CD155 expression. It is up-regulated on endothelia by IFNγ, and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin. On migrating cells, it is concentrated at the leading edge, where it binds basement membrane vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and integrin αvβ3 to promote cell migration. Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration. Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration, but also allows tumor cell recognition and killing by DNAM-1or CD96 expressing NK cells.

Synonym: Poliovirus receptor, CD155 antigen, Nectin-like protein 5, Nectin-2, Tage4 receptor, Pvr, PVR, Necl5, CD155,3830421F03Rik,D7Ertd458e,HVED,mE4,necl-5,PVS,Taa1,Tage4

Molecular Weight: 35.6 kDa

UniProt: Q8K094

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Cancer Immune Checkpoints, SARS-CoV-2 Protein Interactome