REG4 Protein (His tag)

Catalog No. ABIN7197679

Product Details

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This REG4 protein is labelled with His tag.

Purpose: Recombinant Human REG4/RELP Protein (His Tag)(Active)

Sequence: Met 1-Pro 158

Characteristics: A DNA sequence encoding the human REG4 (NP_001152824.1) (Met 1-Pro 158) was expressed, with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions.The ED50 for this effect is typically 4-20 μg/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Alternative Name: REG4/RELP (REG4 Products)

Synonyms: GISP Protein, REG-IV Protein, RELP Protein, 2010002L15Rik Protein, regenerating family member 4 Protein, regenerating islet-derived family, member 4 Protein, Reg4 Protein, REG4 Protein

Background:

Background: Regenerating islet-derived protein 4, also known as REG-like protein, REG4, GISP and RELP, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily, has been found to be involved in malignancy in several different organs including the stomach, colorectum, pancreas and prostate. It is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Expression of the Reg4 in different cell types has been associated with regeneration, cell growth and cell survival, cell adhesion and resistance to apoptosis. REG4 protein overexpression is associated with an unfavorable response to preoperative chemoradiotherapy and may be used as a predictive biomarker clinically. REG4 may play an important role in the development and progression of colorectal cancer, as well as in intestinal morphogenesis and epithelium restitution.

Synonym: Regenerating islet-derived protein 4, Gastrointestinal secretory protein, REG-like protein, Regenerating islet-derived protein IV, GISP, RELP, REG4,GISP,REG-IV

Molecular Weight: 17.4 kDa

NCBI Accession: NP_001152824