SERPINB3 Protein (His tag)

Catalog No. ABIN7197962

Product Details

Target: SERPINB3 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3 (SERPINB3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This SERPINB3 protein is labelled with His tag.

Purpose: Recombinant Human SerpinB3/SCCA1 Protein (His Tag)(Active)

Sequence: Asn 2-Pro 390

Characteristics: A DNA sequence encoding the human SERPINB3 (AAB20405.1) (Asn 2-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenice peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is < 5 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 20 % glycerol, 3 mM DTT

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SERPINB3 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3 (SERPINB3))

Alternative Name: SerpinB3/SCCA1 (SERPINB3 Products)

Synonyms: HsT1196 Protein, SCC Protein, SCCA-1 Protein, SCCA-PD Protein, SCCA1 Protein, T4-A Protein, LEUPIN Protein, PI11 Protein, SCCA-2 Protein, SCCA2 Protein, serpin family B member 3 Protein, serpin family B member 4 Protein, serpin B3 Protein, Somatic cell count Protein, SERPINB3 Protein, SERPINB4 Protein, Serpinb3 Protein, LOC483954 Protein, SCC Protein

Background:

Background: SERPINB3, also known as SCCA-1, belongs to the serpin family. Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. The acronym serpin was originally coined because many serpins inhibit chymotrypsin-like serine proteases. SERPINB3 is expressed in some hepatocellular carcinoma (at protein level). Its expression is closely related to cellular differentiation in both normal and malignant squamous cells. It seems to also be secreted in plasma by cancerous cells but at a low level. SERPINB3 significantly attenuates apoptosis by contrasting cytochrome c release from the mitochondria and by antichemotactic effect for NK cells. It may act as a protease inhibitor to modulate the host immune response against tumor cells and may be involved in the malignant behavior of squamous cell carcinoma cells.

Synonym: Serpin B3, Protein T4-A, Squamous cell carcinoma antigen 1, SCCA-1,serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3, serpin peptidase inhibitor, clade B (ovalbumin), member 3, Squamous cell carcinoma antigen 1,T4-A,SCCA1,HsT1196,SCC,SCCA-1,SCCA-PD

Molecular Weight: 46.7 kDa