SERPIND1 Protein (His tag)

Catalog No. ABIN7197974

Product Details

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This SERPIND1 protein is labelled with His tag.

Purpose: Recombinant Mouse SerpinD1/HCF2 Protein (His Tag)(Active)

Sequence: Met1-Ser 478

Characteristics: A DNA sequence encoding the mouse SerpinD1 precursor (NP_032249.3) (Met1-Ser 478) was expressed, with a C-terminal polyhistidine tag.

Purity: > 98 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit thombin (Sigma, T4648) cleavage of fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catslog# ES011). The IC50 value is < 1.2 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 25 mM HEPES, 150 mM NaCl, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Alternative Name: SerpinD1/HCF2 (SERPIND1 Products)

Synonyms: D22S673 Protein, HC2 Protein, HCF2 Protein, HCII Protein, HLS2 Protein, LS2 Protein, THPH10 Protein, serpind1 Protein, MGC53936 Protein, rls2var1 Protein, AA985900 Protein, AI303446 Protein, Hcf2 Protein, serpin family D member 1 Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade D, member 1 Protein, SERPIND1 Protein, serpind1.L Protein, Serpind1 Protein, serpind1 Protein

Background:

Background: SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Synonym: Heparin cofactor 2; Heparin cofactor II; HC-II; Protease inhibitor leuserpin-2; Serpin D1

Molecular Weight: 53.5 kDa

NCBI Accession: NP_032249