SERPIND1 Protein (His tag)

Catalog No. ABIN7520495

Product Details

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SERPIND1 protein is labelled with His tag.

Purpose: Active Recombinant Human Serpin D1/Heparin cofactor 2 Protein

Sequence: GSKGPLDQLE KGGETAQSAD PQWEQLNNKN LSMPLLPADF HKENTVTNDW IPEGEEDDDY LDLEKIFSED DDYIDIVDSL SVSPTDSDVS AGNILQLFHG KSRIQRLNIV NAKFAFNLYR VLKDQVNTFD NIFIAPVGIS TAMGMISLGL KGETHEQVHS ILHFKDFVNA SSKYEITTIH NLFRKLTHRL FRRNFGYTLR SVNDLYIQKQ FPILLDFKTK VREYYFAEAQ IADFSDPAFI SKTNNHIMKL TKGLIKDALE NIDPATQMMI LNCIYFKGSW VNKFPVEMTH NHNFRLNERE VVKVSMMQTK GNFLAANDQE LDCDILQLEY VGGISMLIVV PHKMSGMKTL EAQLTPRVVE RWQKSMTNRT REVLLPKFKL EKNYNLVESL KLMGIRMLFD KNGNMAGISD QRIAIDLFKH QGTITVNEEG TQATTVTTVG FMPLSTQVRF TVDRPFLFLI YEHRTSCLLF MGRVANPSRS

Specificity: Gly20-Ser499

Purity: > 90 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.The IC50 value is <0.19 nM.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM Tris,150 mM NaCl, pH 7.5.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Alternative Name: Serpin D1/Heparin cofactor 2 (SERPIND1 Products)

Synonyms: D22S673 Protein, HC2 Protein, HCF2 Protein, HCII Protein, HLS2 Protein, LS2 Protein, THPH10 Protein, serpind1 Protein, MGC53936 Protein, rls2var1 Protein, AA985900 Protein, AI303446 Protein, Hcf2 Protein, serpin family D member 1 Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog Protein, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 Protein, serine (or cysteine) peptidase inhibitor, clade D, member 1 Protein, SERPIND1 Protein, serpind1.L Protein, Serpind1 Protein, serpind1 Protein

Background: SERPIND1,D22S673,HC2,HCF2,HCII,HLS2,LS2,THPH10

Gene ID: 3053

UniProt: P05546